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KMID : 0355519880120010013
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Korean Journal of Oral Biology
1988 Volume.12 No. 1 p.13 ~ p.19
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EFFECTS OF cAMP MODULATORS ON THE IN VITRO REACTIVATION OF LATENT HERPES SIMPLEX VIRUS
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정동균/Cheong, Dong-Kyun
박노회/Park, No-Hee
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Abstract
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This experiment was carried out to observe the effects of cAMP modulators i.e., Cholera Toxin (CTX) and adenosine, on the in vitro reactivation of latent Herpes simplex virus (HSV) and CAMP levels in latently infected trigeminal ganglia of mice. In this study, five weeks old of inbred albino male mice were inoculated with HSV1. Four to eight weeks after the viral inoculation, the mice were sacrificed and the ganglion were removed for the study.
To determine the effects of CTX and adenosine on the reactivation of latent HSV1 in vitro, the excised ganglia were organ cultured in Eagle´s MEM containing CTX (3 pg/ml) or adenosine (500 pM) on the replication of HSV -1 were deter-mined using a yield reduction assay.
Cholera toxin increased the rate and number of infectious units produced in the in vitro reactivation of latent HSV, whereas adenosine diminished them. CAMP concentration in latently infected trigeminal ganglia of mice was greatly increased by CTX but was not affected by adenosine.
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